Cutting edge: tumor secreted heat shock-fusion protein elicits CD8 cells for rejection.

Shock-Fusion Protein Elicits CD8 Cells for Cutting Edge: Tumor Secreted Heat

Perforin Is Required for Innate and Adaptive Immunity Induced by Heat Shock Protein Gp96 of CD8 CTL in response to heat shock protein gp96- peptide comlexes, indicating an essential function for perforin-mediated lysis in the nascent NK and CD8 CTL

trauma, infection, or necrosis (Basu et al., 2000; Berwin Tumor-secreted gp96-Ig is highly immunogenic and et al., 2001). Gp96-peptide complexes bind to CD91 and triggers CD8 T cell-mediated tumor rejection. In vivo other receptors on dendritic cells (Basu et al., 2001; secreted gp96-Ig and gp96-myc cause NK activation Berwin et al., 2002; Binder et al., 2000), mediate endocyand clonal expansio...

Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice.

Recently, we reported that heat shock protein 105 (HSP105) DNA vaccination induced anti-tumor immunity. In this study, we set up a preclinical study to investigate the usefulness of dendritic cells (DCs) pulsed with mouse HSP105 as a whole protein for cancer immunotherapy in vivo. The recombinant HSP105 did not induce DC maturation, and the mice vaccinated with HSP105-pulsed BM-DCs were markedl...

Cutting edge: membrane lymphotoxin regulates CD8(+) T cell-mediated intestinal allograft rejection.

Blocking the CD28/B7 and/or CD154/CD40 costimulatory pathways promotes long-term allograft survival in many transplant models where CD4(+) T cells are necessary for rejection. When CD8(+) T cells are sufficient to mediate rejection, these approaches fail, resulting in costimulation blockade-resistant rejection. To address this problem we examined the role of lymphotoxin-related molecules in CD8...

Molecular and cellular requirements for enhanced antigen cross-presentation to CD8 cytotoxic T lymphocytes.

MHC class I-mediated cross-priming of CD8 T cells by APCs is critical for CTL-based immunity to viral infections and tumors. We have shown previously that tumor-secreted heat shock protein gp96-chaperoned peptides cross prime CD8 CTL that are specific for genuine tumor Ags and for the surrogate Ag OVA. We now show that tumor-secreted heat shock protein gp96-chaperoned peptides enhance the effic...